I am trying to analyse the polyphenolic or flavonoid content of my honey sample. Can I do it with some new method that doesn't require purchasing any standard for chemical compounds?
Do you really need HPLC if you wish to get total phenolic or total flavonoid content...simply there are lots of colorimetric assays to do...
Hello.
You can get structural information on PCs by comparing retention times and UV spectra with the literature. Please check the following books:
Campos, M.G., & Markham, K. R. (2007). Structure information from HPLC and on-line measured absorption spectra: flavones, flavonols and phenolic acids. Imprensa da Universidade de Coimbra/Coimbra University Press.
Mabry, T., Markham, K. R., & Thomas, M. B. (2012). The systematic identification of flavonoids. Springer-Verlag.
Regards.
You really don’t need HPLC for total flavonoids and polyphenolic compounds. There are other methods to do it.
Try checking out this paper: Liigand, J., Wang, T., Kellogg, J. et al. Quantification for non-targeted LC/MS screening without standard substances. Sci Rep 10, 5808 (2020). https://doi.org/10.1038/s41598-020-62573-z
you have to do screening to determine the presence of total polyphenol ; you can also get structural information in literature or by LC-MS
If it is total Phenolics no HPLC is needed, check the sent document.Charaterisation would need HPLC
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