Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Spatial Panel Diagnostic Test?

Hello Colleagues Trust you are all fine, Please I have been struggling for days on estimating some spatial diagnostic test on my model. The stata command I am using is spatdiag command. I am...

04 January 2021 3,919 1 View

Can Microplastics act as vector of chemical transport into human system on the model of stomach oil extraction ?

Chemical transport from micro-plastics to animal tissues has been reported in birds.

01 August 2019 9,216 1 View

I need more article on the assessment of nutritional status in school age children?

01 July 2019 6,210 2 View

How do You determine the ranges of value for your parameters, time and temperature using soxhlet extraction(N-hexane And Ethanol) for lemon grassoil?

I'm trying to conduct an experiment on the optimization of extraction of lemongrass oil using soxhlet extraction. My Optimum parameters are temperature, time and lemongrass to solvent ratio. I'm...

08 April 2019 9,256 1 View

How to generate parameters for DNA using Amber?

I am interested in performing MD simulation for one of the DNA demethylase enzymes using Amber. I have been able to generate both the "mol2" and "frcmod" files for the non-standard residues using...

14 September 2018 3,094 2 View

Can I pool sera of chickens within a replicte together for LH FSH ELISA analyses in order to reduce cost of analyses?

I need to run ELISA analyses for reproductive hormones (LH, FSH) in chickens. The samples are however too many. Can I pool sera of birds within a replicate/treatment together for the analyses in...

19 August 2018 9,819 2 View

How can one solve the convergence error in SCC-DFTB QM/MM MD Simulation?

I am trying to run a QM/MM MD calculation on an enzyme substrate system using AMBER after successful completion of MD production run in an Isobaric-Isothermal (NPT) ensemble without errors. I am...

03 May 2018 3,302 3 View

How do I plot my data using the Sigmoidal Langmuir equation?

I've included an image with showing the equation. I need to know what parameters to plot on the x and y axes. Any help will be highly appreciated.

15 January 2018 290 3 View

Which basis set is the most laudable for studying reactions involving halides?

23 November 2014 5,142 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View