After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just bacterial matter?
Of course you will only get bacterial stuff or nothing. I've tried this kind of experiment, and the dirt in the feces is a big trouble affecting the extraction of exosomes. Maybe you can try a rectal rinse, so you may get the exosomes you want. Good luck!