I want to determine the resting spore population present in Barley roots. if anybody can suggest how a single resting spore of Polymyxa graminis looks like, while resting spore suspension prepared by crushing the roots.
There are many methods for Polymyxa graminis detection in plant roots, but you hardly will find large number of spores sufficient for evaluation by optical density. It is easier to stain them or link to specific antibodies and count by number.
http://www.agriculturejournals.cz/publicFiles/63793.pdf
http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2000.90.5.537
http://archive.bio.ed.ac.uk/jdeacon/microbes/chytrid.htm
Thank you Alex Ignatov
Actually I am counting Polymyxa resting spores by using hemocytometer. I crushed root for extraction of resting spore, centrifuged it for getting spore suspension but while i count, i am confused with some small root particles. so is there any way to count it more accurately. i tried staining it with cotton blue, aniline blue, acid fuchsin. but no satisfactory results.
You may treat the roots with GAA for some time and stain them with CB. You may find the good results and observe under microscope.
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