Can we consider hemagglutination assay for dengue virus as a quantitative method???

07 July 2019 1 10K Report

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

Kelli L Barr

HA should not be used to titer dengue virus. You can perform viral plaque assays in Vero cells, rt-PCR (compared with a know viral standard), or you can perform a focus forming unit assay. Some labs use HI but is will only give you a rough estimate of virus. When we have titrated virus by plaque assay and compared it with HA, the results have always been off by at least 1 log in either direction depending on serotype.

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