Can the treatment medium be used in the Griess assay? (H2O2 treatment medium for example)

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Bruce Neagle

The Griess assay requires that Nitrates in the sample be reduced to Nitrites which are reactive. Thus, the continued presence of Peroxide radical is likely to oxidize your Nitrites back to Nitrates. You're better off to use 'ion chromatography' in this case.

Jordana Rodrigues

Well, you have to use the supernatant and that includes the treatment one...

You can do this assay and then a PCR of NOS to confirm your results.