Hi, Elahen, do you mean estimation of the protein amount? Lowry is a method of protein mesurement. For the isolation you can use any method for the extraction.
You have to extract proteins first and then analyse by Lowry metoth
You can estimate protein from virtually anything provided you are able to convert it into an analysable form. In your case, homogenizing seeds is something that could be a challenge. Once properly homogenized in a solution that does not denature protein, you can use any method to estimate protein including Lowry's method. Do not forget to take into consideration the dilution factor (amount of homogenising liquid used/g of seed) while calculating. Normally protein estimation is given in gram/deciliter. In your case you can convert it into mg/g of seed. Best Regards.
Dear Elaheh
first you should extrction protein with chromatography method.there after lowry or bradford,estimate concentration of protein.anyway lowry is more accurate than bradford.
dear college:
what are your seeds belong to?
Several extraction methods can be carried out if the seeds are known
Dear sou fain
Did you mean for estimation of protein quantity or isolating protein from seed , if you are concentrated on isolation of protein you can use any extraction buffer (Daily using) it containing cysteine or beta merkeptaoethanol.
If you are doing estimation only better to go for bradford's reagent assay, lowry is also a batter method but as per my knowledge you are finding phenolics can interfere.
All the Best
I endorse dr navin rajesh recommendations, however, in the Lowery method soluble proteins are mostly estimated, therefore, making homogenate in bi distilled water is also workable.
Thank you everyone.
But I have problems to find a solution for protein extraction because most methods are expensive!
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