Liposome (or proteoliposome) filed is new to me. I do not know this procedure to produce liposome make sense. What I did is prepare DMPC liposome with and without 100mM magnesium. The results were interesing. DMPC in 20mM Tris (7.5), 20mM NaCl, gave kind of transparent solution, still not sure there is liposome or not. DMPC in 20mM Tris (7.5), 20mM NaCl, 100mM MgCl2, gave milky solution. Do you think I have liposome with both procedures. By the way, I prepared liposome with biobead methods. 200ul of 20mg/ml DMPC in 100mM Sodium cholate, 20mM Tris, 20mM NaCl, and (100mM MgCl2) was applied to 120mg of washed biobead SM-2 and incubated 2 hours at 4'C rotator. After recover solution, I did it again with 80mg of washed biobead SM-2 again with the same way.
I want to ask this procedure makes sense for liposome experts.