A Polymeric thin film is been made by drop casting method on the stainless steel substrate. Now I need analyze the produced thin film by SEM. So can I use this one, I mean the steel as a substrate of mentioned polymeric thin film, in SEM?
The steel is conducting which you could be used as a substrate of your polymeric thin films for SEM. However, you may have some features of your steel substrate on the SEM images. You could try that. Ideally your polymeric thin films should coat on a gold covering glass substrate or a silicon substrate. It is much easy to analyse the SEM images of your polymeric thin films.
As stated by Hassan Sarigul, you need gold or Pd coating before SEM analys. But taking the interface scans may be bit difficult. If it is a latest SEM of jeol make, they have provided some adjustments to take such scans, Please look into the product catalogue of JEOL SEM.
While gold or platinum substrates are better, the stainless steal in most cases is enough conductive for SEM. Moreover, if the task is to study the polymer thin film on the stainless steal substrate its usage is preferable as nature of the substrate influences morphology and structure of the precipitated polymer film due to influence of interactions on the interface and even to wettability of the substrate by the solvent . So, you can get different surface of the thin polymer film on the different metals.