Can someone tell me what software/s I can use to compare virulence and resistance genes from my WGS file (contigs) with other closely related strains?

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- Can anyone tell me how to fill gaps in a draft genome assembly?

- I mapped my whole genome against the reference strain, and found 100 bp gaps between all the contigs? Is it something to be worried about? How can I overcome this shortcoming in my sequence?

02 March 2018 1,188 16 View

Can anyone tell me why the macrophages or monocytes are used to study intracellular survival of pathogens?

- Uropathogenic E.coli is an intracellular pathogen and invades bladder epithelial cells lines. I have come across numerous research papers where researchers have used bladder epithelial cells...

01 February 2018 3,136 3 View

Usually how long the mammalian cell lines are incubated in 24 wells TCP (seeded with 5x10^5 cells/well), in order to perform invasion assays?

- I seed 24 wells TCP with a seeding density of 5x10^5 cells, and let them grow for 24 h.After 24 h they reach a cell density of approximately 70-80%. I infect them with my bacteria, and after...

01 February 2018 6,183 3 View

Hello everyone! I am naive in NGS/bioinformatic. Can anyone tell what does it mean when the company says 1 of your strains has less than 30X coverage?

- Since I have very less background in bioinformatics, I find it hard to understand some of things. Looking forward towards kind replies. Thanking in anticipation :)

31 December 2017 4,723 5 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Hi i m workiking with hct116 resistant and naïve cells after passage half of my cells die can anybody help?

thanks

25 June 2024 4,667 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 4,763 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 6,583 2 View

Effect of post bake on PPR resist?

HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...

18 June 2024 5,741 0 View

I am working on a virulence genes in staphylococcus and i need paper on previous and similar works?

i am working on a virulence genes in staphylococcus and i need paper on previous and similar works

17 June 2024 5,534 2 View