- Can anyone tell me how to fill gaps in a draft genome assembly?

- I mapped my whole genome against the reference strain, and found 100 bp gaps between all the contigs? Is it something to be worried about? How can I overcome this shortcoming in my sequence?

02 March 2018 1,099 16 View

Can anyone tell me why the macrophages or monocytes are used to study intracellular survival of pathogens?

- Uropathogenic E.coli is an intracellular pathogen and invades bladder epithelial cells lines. I have come across numerous research papers where researchers have used bladder epithelial cells...

01 February 2018 3,061 3 View

Usually how long the mammalian cell lines are incubated in 24 wells TCP (seeded with 5x10^5 cells/well), in order to perform invasion assays?

- I seed 24 wells TCP with a seeding density of 5x10^5 cells, and let them grow for 24 h.After 24 h they reach a cell density of approximately 70-80%. I infect them with my bacteria, and after...

01 February 2018 6,091 3 View

Hello everyone! I am naive in NGS/bioinformatic. Can anyone tell what does it mean when the company says 1 of your strains has less than 30X coverage?

- Since I have very less background in bioinformatics, I find it hard to understand some of things. Looking forward towards kind replies. Thanking in anticipation :)

31 December 2017 4,632 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View