Hi

Look into attached pdf, this might help you.

Hi

enclosed is a method

https://www.researchgate.net/publication/45493325_A_new_microtitre_plate_screening_method_for_evaluating_the_viability_of_aerobic_respiring_bacteria_in_high_surface_biofilms

Article A new microtitre plate screening method for evaluating the v...

Dear All,

Thank you for your replies !

To evaluate the antibiofilm efficacy of different matherial such as titanium surfaces, the whole biomass present on each disc was detected. Briefly, biofilms grown on titanium

discs were air-dried and stained by disc immersion in a 5% crystal violet solution for 15 minute and, after several washing , air dried again. The estimation of biofilm biomass was performed by elution of the biofilm bound crystal violet with ethanol (96%)

followed by the determination of the absorbance of 100 ll of eluted dye solution at 595 nm using a microplate photometer (Multiskan FC, Thermo Scientific; Milan, Italy). Measurements for each discs, were carried out in triplicate. For each bacteria and

surface, mean values and standard deviation of absorbance value were computed. Un-paired Student’s T test was used to compare data between experimental surfaces.

I hope this protocol help you

Although it is in Spanish I hope to help you.

http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S1316-71382016000100006

Thanks Mehdi Rostami Rad  and Marielsa Gil.

First prepare biofilm of  Staphylococcus and confirm its stability by measuring size by electron microscope. Now treat with supernatant  at different time interval and check the strength and thickness using electron microscope or by the method mentioned by Dr Mehdi Rustami. You will certainly find some effect on biofilm. 

 Refer this article

Article Coral-Associated Bacteria as a Promising Antibiofilm Agent a...

Thanks Murugesan Sivaranjani and Anita Sharma.