Can someone suggest a way to isolate murine monocytes from bone marrow?

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Sławomir Lewicki

Ficoll (or something similar ) - mononuclear cell isolation,

then monocytes isolation - e.g. immunomagnetic column-free cell isolation or cell sorting by flow cytometry

Raghav Oberoi

Can I use adherence technique, like for human monocytes ? I don't want to go for magnetic beads.

Prahlad Parajuli

Yes, adherence technique will work fine. Incubate the bone marrow cells for 1-2 hours at 37 degree to let the monocytes adhere.