Can someone suggest a lysis reagent/protocol suitable for 96-well yeast lysis which is compatible with protein assay kits?
I want to assay the activity of antioxidant enzymes in the yeast Candida albicans using commercially available assay kits. I would like to lyse the yeast cells in 96-well plate format and am looking for a simple way to do this. I cannot use beads as I do not have the equipment. I have been looking around at various lysis reagents online but many of them have detergents - the kits I want to use to assay enzymatic activity have not been tested in combination with detergents so I would ideally like to gently lyse my cells without detergent. Is this even possible?
Thanks in advance,
Karen O'Hanlon Cohrt PhD
Thanks Helena, I only seen your response now. I have looked around online and found a few kits but when I asked the provider for support the standard answer is that the lysis kit/reagant in question hasn't been tested in combination with the biochemical assay I am trying to run. I will probably end up taking a chance on one of these kits. Thanks for answering :)
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