I would like to know, if any one can suggest a protocol for developing such construct. I also like to know if anyone can suggest probable outcome, can it be fruitful?
There are already several vectors based on Gateway technology where you can introduce your sequence of interest and allow the production of hairpin RNA. Just make sure your sequence is specific for your gene of interest, unless you want to silence several related genes: http://gateway.psb.ugent.be/search/index/silencing/any
If you are interested on the basics of the system and some considerations to take into account, check: http://www.benthamscience.com/open/topsj/articles/V002/21TOPSJ.pdf