Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Reason for "reading bands namelist error" in band structure calculation in quantum espresso?

At first, I did the scf run then nscf run followed by another run with calculation set to "bands'. And finally, using command bands.x < example.in > example.out to post-process the data, I...

10 February 2021 7,970 2 View

How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Why the bandgap of CH3NH3PbCl3 films are different for spin-coating method and electrospinning method?

The observed bandgaps are 3.8 eV and 3.4 eV for spin-coated and electrospun films, respectively. What are the possible reasons?

08 January 2021 5,646 6 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to select the active and inactive sites for CASSCF calculations?

I want to do CASSCF calculations for transition metal dimers (eg. Mn2, Cr2, Cu2). Want to optimize and see the energy. Can anyone send me the input file for gaussian.

24 October 2019 6,124 1 View

How to select the active and inactive sites for CASSCF calculations?

I want to do CASSCF calculations for transition metal dimers (eg. Mn2, Cr2, Cu2). Want to optimize and see the energy. Can anyone send me the input file for gaussian.

09 October 2019 8,686 1 View

CASSCF calculation worked but did not show the final one electron matrix. Can I get it from the chk file?

Hello everyone. I am performing a casscf calculation of an organic molecule containing 43 atoms in gaussian 09 (rev. D01), and realized that the job ended well, but did not showed the one...

20 January 2019 9,701 3 View

How is performed the orbital optimization for a CASSCF calculation GAUSSIAN without the keyword 'stateaverage' ?

Hello, I am mainly doing CASSCF calculations with MOLPRO, but I want to do the equivalent with GAUSSIAN. I think that in MOLPRO, both the ci and orbital coefficients are optimized together to...

31 December 2018 3,391 3 View

Final one electron symbolic density matrix - does it describe the highest excitation state?

I am trying to calculate some excitation states using Gaussian. When i run the CASSCF calculation in gaussian with nroot option (it gives opportunity to explore excitation states) - the Final...

10 November 2017 167 0 View

How to make an input file for CASSCF single point energy calculation for a molecule at triplet state (T1) in gaussian 09?

I want to calculate the single point energy of a molecule in the T1 state using CASSCF. Can anyone post a test file for the above mentioned calculation? The molecule is more stable at S0.

20 June 2017 8,632 3 View

Is it possibe to compute avoided crossings between triplet and singlet surfaces using CASSCF?

I conducted a scan in the ground state using M06-HF functional and cc-pVDZ basis set on the system which I am working. Then TDDFT single point energy calculations of the scanned structures were...

12 June 2017 4,348 4 View

Is it possibe to compute avoided crossings between triplet and singlet surfaces using CASSCF?

I conducted a scan in the ground state using M06-HF functional and cc-pVDZ basis set on the system which I am working. Then TDDFT single point energy calculations of the scanned structures were...

05 June 2017 1,353 5 View

How to make an input file for CASSCF single point energy calculation for a molecule at triplet state (T1) in gaussian 09?

I want to calculate the single point energy of a molecule in the T1 state using CASSCF. Can anyone post a test file for the above mentioned calculation? The molecule is more stable at S0.

05 June 2017 9,872 3 View

How to do CASSCF/CASCI calculations ?

How to do CASSCF/CASCI calculations to investigate the electronic state of metal atom presented in octahedral arrangement.

10 November 2016 7,180 0 View