Using pot experiment in a screen house rice varieties of rice was grown in soils that are highly toxic in Iron
heavy metal toxicity can be analysis by using antioxidants enzymes or by icp-ms techniques.
can you please explain more about your queries
Iron toxicity in rice can be measured by various methods. ICP-MS is expensive but may provide quick and best result.
Bipyridine method in which spectrophotometer is use also use widely for such purpose.
Go for antioxidant enzyme activity determination (SOD, POD, GPX etc. activities) using spectrophotometer or colorimeter.
Iron is involved in the production of ROS by means of the Fenton reaction, so you can assayed oxidative stress markers. Perls staining also revealed the accumulation of iron in the tissue and cells.
You can qualified the present of iron in the shoot or root by 2,2 bypiridine
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As suggested above, you can go for various methodologies to quantify the toxicity qualitatively as well as quantitatively.
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