Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000?

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000? If yes- Is there some maximum number of bp of RNA, which could be transfected using Lipofectamine 3000? I...

08 November 2018 9,558 3 View

What is the optimal concentration of miRNA for microinjection into porcine oocytes?

Our stock is 5nmol. Or is it different for each miRNA? Thank you for answers.

30 May 2018 6,655 2 View

Someone who is able to statisticaly analyze MALDI-TOF data?

I am looking for anyone with experience with statistical comparison of MALDI-TOF data. I have no experience with analyzing profile data and I need to compare the effect of the treatment on...

19 September 2017 5,091 3 View

How to define multi volume heat fluxes within DFLUX subroutine, Abaqus?

24 May 2017 1,673 1 View

Why reporters of deviations in pharmacotherapy are not established and well paid in many European hospitals?

According to the media many fatal medical errors have been happened in many European countries. Although a 'soul' of inappropriate treatment and prescribing is well known in the literature, in a...

31 May 2016 2,834 8 View

Why and when should we combine or switch antidepressant in patients with major depressive disorder MDD?

02 January 2016 8,597 18 View

Why many Eastern European countries did not establish clinical pharmacy service in the hospitals next to the patients? Who will deal with errors??

According to the newest evidence more than 10 % of patients are admitted to the hospital, because of inappropriate prescribing and drug-related problems. More than 20 % of patients are treated...

11 October 2015 8,973 57 View

What is your opinion about quality of antibiotic prescribing within psychiatric wards and hospitals?

Prudent prescribing of antimicrobial drugs to hospital inpatients may reduce the incidence of antimicrobial drug resistance and healthcare-associated infection. Despite strenuous efforts to...

24 March 2015 3,714 14 View

Are there any studies showing age related hypertension in regular strains of mice?

In particular I'm interested if hypertension occurs in C57BL6 mice at any time after their 18th month of age. But I am also interested in other strains. If you could point me to such studies I...

04 March 2015 401 6 View

Can anyone offer us analysis of homoarginine?

We have introduced the methodology for the estimation of reactive lysine in our laboratory. In the last step determination of homoarginine is necessary. Can anyone offer us this analysis?

01 February 2015 9,714 4 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Wind speed Forecasting: Training set size?

I have one year of wind speed data, collected at hourly intervals. I am trying to train a forecasting model using this data. I want to be able to generate rolling forecasts up to 7 days in the...

23 January 2021 3,313 5 View

I need a Wind Power Dataset having wind turbine's output power values and corresponding weather data information: wind speed, pressure, temperature?

The dataset is needed for forecasting wind power output using the weather information. I will be much obliged if anyone can share a relevant dataset or link.

04 January 2021 6,344 2 View

Does anyone have an insight into the behavioural response of fishes to moving wind turbine blades as seen through the air-water interface?

I am interested to find out if wind turbines located close to rivers or lakes, or perhaps even in marine locations, are a potential problem for fish. This seems to be a very under-researched area...

04 January 2021 3,468 2 View

How can help me to Convert the files DIgSILENT PowerFactory example to an earlier version (v15.1.7)?

Hello , How can help me to convert this example files Power Factory version ,The files example from the DIgSILENT website has been created with version 15.2.5 which I do not have access to. I'd be...

31 December 2020 4,213 1 View

Why am i getting a negative torque in ansys fluent for a vertical axis wind turbine?

I am trying to simulate a vertical axis wind turbine of darrieus model and i am getting a negative torque. I have followed frame motion, relative to adjacent cell method, and solution method as...

28 December 2020 1,170 3 View

How to identify missing /bad data in PMU data?

PMU data can go missing due to several factors - faulty PMU or PDC or communication channel, cyber attack, etc. Bad data can be also caused due to some of the above reasons or maybe due to some...

28 December 2020 8,237 3 View

Please share tutorial/literature about dsl modelling and composite model (digsilent powerfactory)?

please share tutorial/literature about dsl modeling and composite model (digsilent powerfactory)?

22 December 2020 3,319 2 View

Where Can I Get Hourly Wind Speed Data at 50 Meters Height?

Hi, I want to get three years of hourly wind speed data at 50 meters height for specific locations in Turkey. Unfortunately, I couldn't find any good websites for this. Do you know any online...

12 December 2020 323 10 View

Is it possible to simulate a environment of energy system combine with Solar, wind and traditional energy sources. Please suggest me some sof. too?

Is it possible to simulate a environment of energy system combine with Solar, wind and traditional energy sources. Please suggest me some software too. Even the energy conversation, efficiency...

04 December 2020 1,088 13 View