Saurabh,

Here are some possibilities:

Coumarin 153 in methanol, excitation wavelength 295-442 nm, lifetime 4.3 ns.

reference: Boens, et al. (2007) Anal. Chem. 79: 2137-2149.

Coumarin 540 in acetonitrile, absorption maximum 454 nm, lifetime 3.2 ns.

reference: Jones, et al. (1985) J. Phys. Chem. 89: 294-300.

Coumarin 535 in ethanol, absorption maximum 433 nm, lifetime 3.2 ns

reference: 

DCM in acetonitrile, absorption maximum 460 nm, lifetime 1.9 ns.

reference: Bourson and Valeur (1989) J. Phys. Chem. 93, 3871-3876.

Perylene in cyclohexane, absorption maximum 436 nm, lifetime 4.7 ns.

reference:  Berlman (1965) Handbook of Fluorescence Spectra, Academic Press.

Auramine O in 1-decanol, absorption maximum 435 nm, lifetime 92 ps.

reference: Gautam and Harriman (1994) J. Chem. Soc., Faraday Trans. 90, 697-701.

Acridine orange in water, absorption maximum 450 nm, lifetime 1.7 ns.

reference: Kubota and Steiner (1977) Biophys. Chem. 6: 279-289.

I hope this some help.

Cheers,

John

Coumarin is a popular dye that absorbs at wavelengths around 400nm. See, http://dx.doi.org/10.1021/ac062160k .

Saurabh,

Here are some possibilities:

Coumarin 153 in methanol, excitation wavelength 295-442 nm, lifetime 4.3 ns.

reference: Boens, et al. (2007) Anal. Chem. 79: 2137-2149.

Coumarin 540 in acetonitrile, absorption maximum 454 nm, lifetime 3.2 ns.

reference: Jones, et al. (1985) J. Phys. Chem. 89: 294-300.

Coumarin 535 in ethanol, absorption maximum 433 nm, lifetime 3.2 ns

reference: 

DCM in acetonitrile, absorption maximum 460 nm, lifetime 1.9 ns.

reference: Bourson and Valeur (1989) J. Phys. Chem. 93, 3871-3876.

Perylene in cyclohexane, absorption maximum 436 nm, lifetime 4.7 ns.

reference:  Berlman (1965) Handbook of Fluorescence Spectra, Academic Press.

Auramine O in 1-decanol, absorption maximum 435 nm, lifetime 92 ps.

reference: Gautam and Harriman (1994) J. Chem. Soc., Faraday Trans. 90, 697-701.

Acridine orange in water, absorption maximum 450 nm, lifetime 1.7 ns.

reference: Kubota and Steiner (1977) Biophys. Chem. 6: 279-289.

I hope this some help.

Cheers,

John

Hey Christoph!

Thanks for your reply! The two fluorophores that you mentioned; do they absorb light at 440nm? Because that is my limitation. Thanks,

Saurabh