Can somebody share a protocol on how to do Environmental Scanning Electron microscopy on culture cells grown on silicon chips.

I want to do immuno labelling in  SEM using gold label secondary antibody for which my query is

A) What kind of fixative should I use?

B) Is silver enhancement necessary if I use gold labelled  Antibody of 10nm?

C) Is any additional coating required e.g. like chromium/Platinum for ESEM ?

look forward to hear from you soon.