How do i prepare rat colon samples for ANTIOXIDANT assay?

I stored small pieces of rat colon in PBS at -20 and now I want to do SOD and MDA assay, I also need to do the Bradford assay to quantify the protein content in the samples. can anyone tell me...

07 January 2021 2,488 1 View

Please I was wondering if anyone could show me a good online calculator for combining mean and SD for my meta-analysis?

03 October 2017 2,846 6 View

Is it possible to predict liquid that can drop out of a gas?

if so how do i know what volume is hydrocarbon and which is water?

13 April 2016 3,923 2 View

Does anyone know of a kit that can be used to measure in vivo vitamin production?

It is known that beneficial bacteria within the colon produce vitamins and consumption of prebiotics enhances the growth of these beneficial bacteria. I am administering a test prebiotic to lab...

29 October 2013 8,389 1 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Age of the Isimila Prehistoric Site in Tanzania?

Does anyone knows results from the new dating program from the Isimila Prehistoric Site in Tanzania?- of course without to give away too much, before a final publication. The data of ca 260...

03 February 2021 6,864 3 View

What if there is no population to pilot an adapted questionnaire? What about its reliability and validity?

Hello, I'm new to this platform, and this is my first ever post in here. So, please excuse me in advance if I happen to cause any inconvenience. Coming back the question; I have just adapted a...

26 January 2021 9,030 4 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View