I just used Kinovea to measure the trajectory of the hand in the videos of the experiment with dancers. If you use the calibration properly, knowing well one of the real distance of the image, then you can use it to meassure inside the picture.
You can also try Aphelion (commercial software, but with a free 30 day evaluation period). With it, you can define the calibration and make the calibrated measurements interactively.
Hi, try imageJ. If i'm correct that can measure distance between two points with translate pixel distance to mm (for example from dicom tags).
Otherwise: you can translate this if and only if, when you have a pixel spacing or can you calculate this from image (e.g. you know what is real size of some object / strucutre into image).