Hi,
I want to stain brain sections of mice which have been xenografts with tumor and treated with a new anti cancer agent. Can some please share the protocol with me including tips and does and dont's for the staining. I would also appreciate cat# for antibodies and any other critical reagent.
Hello Shashank,
We got favorable results with this one:
Bethyl laboratories IHC-00059, rabbit anti-gH2AX. The protocol we used is described in the following publication:
https://www.thegreenjournal.com/article/S0167-8140(20)30104-3/fulltext
In case you don't have access or need more details, please feel free to contact me.
If you are interested in counting gH2AX foci, you might consider using a tyramine super boost kit (e.g. from Thermo) for signal enhancement.
In case you want some evaluation approaches, we have several automated scripts for gH2AX evaluation.
Best wishes,
Theresa
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