How can I avoid signal loss after long polyA/polyT repeats in sanger sequencing?

01 February 2015 9,880 5 View

How to correctly calculate melting temperature and annealing temperature of primers?

There are many formulas and program applications to compute melting temperature and annealing temperature of PCR primers nowadays, but these tools usually have different results. Would you be so...

02 March 2013 238 26 View

How to convert FASTQ format to FASTA on the local computer under Windows operation system?

How to convert FASTQ format to FASTA on the local computer (without Internet) under Windows operation system? Is it necessary to use another operating system?

01 February 2013 5,808 4 View

What is the best tool for comparing and aligning large sequence fragments?

I need to compare some sequences longer than 10Mb. What kind of software is the best for such work? Can I do this work locally (without internet sources)? If I have sequences in FASTAQ format, I...

31 December 2012 9,745 7 View

Phylogenetic markers selection for distinguishing taxa in plants

We work with one genus Betula on the territory of Ukraine. We try to diverse different taxa in this genus based on molecular markers. But we have a problem, that traditional molecular markers...

11 December 2012 3,605 41 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View