after peptide synthesis then need use enough tfa cooktail to cleave the peptide from the resin. and how to easy separate the peptide and tfa is really bored work. especially when use much tfa.
can someone introduce any adsorbent easily extract the peptide from tfa cooktail ?
Have you tried to precipitate the peptide with e.g. Et2O? This procedure removes any residual TFA, except stoichimetric counterions.
Otherwise, SPE or HPLC purification (RP or IEX) should help.
Using an Fmoc based synthesis strategy instead of Boc is another option. Then you have to deal with Piperidine.
thank you very much.I now just use Et2O to precipitate the peptide .but now I synthesize much quantity peptide in lab and need use more and more Et2O,and Et2O is very dangerous in lab so Iwant to find some adsorbent to see if can directly adsorb peptide from TFA ？ can have some ideal?
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