I am preparing a culture media containing nutrient agar, ammonium sulfate and 0.13M tris buffer pH9 solution, for a ureas active bacteria where its optimum growth pH is 9. I read in a paper in which they used sodium acetate as pH stabilizer instead of tris buffer. Can adding sodium acetate act as pH stabilizer?
The urease active bacteria tend to increase pH due to ammonium ion production when grows. So pH of media is gradually getting increased by bacterial growth. Can sodium acetate act as neutralizer of increased pH in environment?
Buffer Reference Center
http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html
Thymol Blue solution is good to stabilized solution at pH 9 . Details are available in literature.
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