How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

Can I know how to apply contourlet transform for signals in MATLAB?Must I first convert 1D signal to 2D image in order to do this method?

01 February 2021 3,735 3 View

Which factors contribute to instructional resilience in HE?

If you are teaching in Higher Education, please consider participating in our study on Instructional Resilience during Emergency Remote Teaching. We want to learn about which factors have...

19 January 2021 6,759 15 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How to compute the phase from a fringe pattern using Hilbert Huang Transform in MATLAB?

I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...

10 January 2021 5,036 1 View

Which statistical test is best to run on a heavily right-skewed dependent variable?

My dependent variable when graphed is heavily right-skewed. All attempts to transform it has failed (Log, Log10, Sqrt, ..). The most ideal test I would like to run is a multiple linear...

07 January 2021 2,705 4 View

How can I estimate and plot Logarithmic Error Bars (in Excel, preferably)?

Dear Dr.colleagues, I am currently plotting the dynamics of an infection in Excel. For each sampling day, I plotted the mean infection load of the population, using the standard error for the...

07 January 2021 2,069 3 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View