Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Forced Vibration Testing using FDD?

Hi, Can we use FDD algorithm for forced vibration response (sinusoidal response produced from eccentric mass vibrator)? Thanks

22 January 2021 5,851 2 View

How can I simultaneously recognize unknown patterns and variables from a given data set?

20 January 2021 6,385 3 View

Can I use breaking series as a regressor in a VAR model?

Context: I have two series, price and sales. Sales is mean-reverting stationery but price is stationary only after controlling for an intercept break. I want to set up a 2-equation VAR model...

14 January 2021 6,824 12 View

What should my objective ?

I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks

13 January 2021 4,635 6 View

How to use Cantera's equilibrium program to calculate adiabatic temperature of dust flame?

To calulate the adiabatic flame temperature using Cantera, we need to provide a .cti file that stores all thermodynamic information needed. We can simply convert a Chemkin mechanism file to .cti...

13 January 2021 7,072 2 View

What is your philosophy about life ?

how do you understand life as it is really big suspense, why we are here, our consciousness is big unconsciousness and our unconsciousness big consciousness.

25 December 2020 7,632 19 View

What is the essence of bio electricity inside us ,why it get resonate during meditation ?

Many time during meditation whole body feels the flow of electricity like the thing is it bio electricity or something else why this occurs but after that generally, I felt so miracle...

25 December 2020 3,754 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Which compound is getting oxidized during bulk electrolysis with TFAA?

The experiment is really simple. When I do a bulk electrolysis with 1% TFAA (ACN solution) to get dihydrogen, what is going to be oxidized? (working electrode graphit, counter electrode platinum,...

18 February 2021 4,430 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View

Are there any thermochromic materials that reflect infrared light (~9000nm) at 10C and transmit infrared light at 25C?

So I know some metal-insulators (ex VO2) have thermochromic infrared-reflective properties, but most of these are insulators at low temperatures and conductive at high temperatures. Some...

07 February 2021 1,770 3 View

How do I analyse a Photoluminescence Spectrum ?

I have a few questions related to PL (images attached for same material by 2 methods), It would be great to get some help. 1. What is the physical meaning of highly intense PL? Does this mean...

02 February 2021 1,791 3 View