Mineral formation in much larger and more rapidly growing species than mice may indeed require the annexin A5, annexin A6 and type X collagen. It is imperative that larger species than mice be used because one of the major things we have observed is that an increase in the rate of mineral formation is the primary effect of the annexins seen in our studies. These have been discussed in detail in my in depth review on MV published in Frontiers of Bioscience 16: 2812-2902, 2011. The presence of annexin A5 as one of the major proteins in MVs isolated from rapidly growing chickens, its ability to form nucleationally active complexes with phosphatidylserine-Ca-Pi complexes, etc., etc., clearly indicates its functional involvement in MV mineralization. I would welcome a thorough discussion of this area.

Article Depletion of annexin A5, annexin A6, and collagen X causes n...