How are you gonna feed your sample to the MS? What compounds are you interested in analyzing? Hydrophilic? hydrophobic?

Hi Omar,

I'm looking at organic acids and I'm using an Aminex column to separate them before detection on the MS. 

If you inject a sample without treatment to the column and your mobile phase is aqueous, I suppose that the tyloxapol will be retained more than the organic acids. The separation of the acids will also be perturbed but you can give it a shot.  

If you want to remove tyloxapol a priori I would run either reversed phase or solid-phase extraction using silica C18 to adsorb the surfactant while the organic acids elute using an aqueous buffer at pH above the pKa of the organic acids. If the organic acids are small I think you'll be fine.