I have made an attempt to detect nitrogen using GC with argon as a carrier and TCD as a detector. I have compared my samples using an analytical grade std. N2 gas. The gas samples were collected from a bioreactor where N2 is the terminal product.
It is very simple to determine N2 in a gaseous sample, you may determine a number of other components in your gas sample from a bioreactor /O2, CO, CO2, CH4 ,H2S, H2O......../.
Instead of the FID use TCD /thermal conductivity detector/, and an optimal carrier gas in your case is He of H2. If you might be intertested in hydrogen content, too, use rather expensive He. In most of gaseous samples analyses the column temperature is moderately higher as that ambient, however in complicated cases the programmed increase in the temperature is advisible.
As a filling of the column you may chose some of the molecullar sieves /e.g. Molecular sieve- Linde 5x, Chromosorb 102 and other syntetic fillings provided by any commercial firm dealing with chromatograpghic suplies/. Check the corresponding catalogies of Merck, Lambda and many others.
Article: Simultaneous Determination of Ne, Ar, and N2 in Groundwater by Gas Chromatography
Pawel Mochalski, ireneusz sliwka, Jan Lasa
Chem. Anal. (Warsaw). 01/2006; 51:825-831.
https://www.researchgate.net/publication/239730712_Simultaneous_Determination_of_Ne_Ar_and_N2_in_Groundwater_by_Gas_Chromatography?ev=prf_pub
Article Simultaneous Determination of Ne, Ar, and N2 in Groundwater ...
Dear Colleague, About denitrificatio problem in grounwater estimation please ask dr Mochalski Pawel, he has some experience in this fields.
The GC method described in my answer is OK, I used it many time when analysing gases arising during wood thermal decomposition /pyrolysis/.
Kjehldal metohod is used for N determination in solid, and maybe some high boiling point liquid samples.
Best regards Nikhil,
R.S.
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