I am planning to use Vybrant cell stains with IF on adherent cell culture. I think that I need to do Vybrant staining first, and continue with fixation and IF. Is it possible? Besides, can I take images when I store the slide at +4/-20oC?
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05 November 2023 2,247 2 View
I want to learn about Solving Differential Equation by using "Discrete Singular Convolution"method. I want to learn this method, if someone has hand notes it would be great to share with me. I...
20 May 2022 2,148 1 View
In angiosperms, they have double fertilization event. Firstly, pollen goes to stigma (pollination). Following pollination, the pollen tube enters an ovule through the micropyle, and then proceeds...
14 April 2016 3,303 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
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08 August 2024 1,514 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
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07 August 2024 1,706 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
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07 August 2024 2,239 5 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View