I had frozen samples that I used for the Spring 23 semester in a -80 degree freezer on campus. Some time between May 5th to now something happened to the freezer and everything in it thawed out; and all my samples have grown mold on them. We have some of the same samples, just from a different location, dried out in our herbarium on campus. Would I be able to use those in place of the frozen samples I lost? If so, how much would the whole process vary? Would I be able to use the same protocols as if they were my frozen sampels?
You can't use these samples because they are from different location even its the same sample also the lost samples are frozen and these samples are not all these reasons give you different results
All the conditions in bringing out the experience must be equalized.
DNA extracts from herbarium samples were quite difficult and we need modified protocols Like PTB/DTT(Phenacylthiazolium bromide/dithiothreitol) and CTAB method and DNeasy Plant mini kit(Qiagen) and the same problem prevails for AFLP also. The protocol is based on the kind of plant material we are using to extract the DNA. The old and degraded samples have lower DNA yield. However, there are several factors like green, pale yellow, brown and dark brown to black that influence the selection of herbarium specimen for the DNA extraction. But the main factor that we considered was the intensity of the green colour of specimen for good quality of the DNA.
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