Hi, Can I use the same kit (Salivary cortisol kit, Salimetrics assay) to measure the cortisol level of saliva and hair?
You must consider detection range as well as extraction procedure. These are two critical points in such an assay
Try to extract the steroids from hair using any standard organic phase extraction procedure. Then, use the kit for saliva
I am working on a project to integrate data from Campus Management Systems and Learning Management Systems into a predictive AI model that forecasts students' academic performance. I want to use...
22 June 2024 831 2 View
I have run MD simulation of a protein-protein docked complex for 50ns. The RMSD plot obtained from the simulation is attached here. The RMSD plot shows a stable trajectory for entire simulation,...
09 June 2024 7,886 1 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
01 June 2024 894 1 View
after defining models structure, the created zones were dispayed in 3D Viwer. then from a right click, cutaway properties was used to get the slice/cross section. Unfortunately, the zones are...
20 December 2023 2,638 0 View
how to adjust Signal Amplitude and Frequency Range so as not to have an overload result in EIS measurement for chitosan membrane?
11 October 2023 9,198 3 View
In the study of quantum deformations of Witt and Virasoro algebras, the notion of a Hom-Lie algebra first appeared, so is deformation theory insufficient to justify how algebraic structures such...
20 September 2023 3,534 1 View
Giuseppe Piano was the first to establish the axioms of vector space, where the group axioms were linked to the axioms proposed regarding the second law, “number multiplication,” with a basic...
15 September 2023 4,234 1 View
Following the findings in the field of deformable algebra, can deformation of an n-dimensional vector space contribute to changing its initial dimension? How will this contribute to finding the...
08 September 2023 7,410 3 View
Hi. I am working on drought prone areas and wants to calculate the 75% of dependable rainfall but i am unable to find the formula.
20 August 2023 5,584 3 View
What are the best programs / softwares for studying the chemical-physical properties interactions between montmorillonite and anionic polymer
26 March 2023 7,136 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....
05 July 2024 5,860 1 View