Hello everybody!

I want to use for the analysis of Chlorella vulgaris extract method Zcillner and. Kirsch (1962). This method is used for analysis of blood serum total lipids. I read that vanillin-phosphoric acid reagent reacts only with the decay products of unsaturated lipids (Lipids + sulfuric acid were placed for 20 minutes in a water bath = decomposition products of unsaturated lipids).

But in the blood (and chlorella - consisting of chlorella about 80% unsaturated fatty acids) have lipids myristic, palmitic and stearic acids saturated, these lipids are not taken into account when applying this method?

(an example of using this method in the attached article)

Thank you!  

http://www.biodiversitylibrary.org/content/part/JAMCA/JAMCA_V01_N3_P302-304.pdf