Fungal strains are Botrytis cinerea، Penicillium expansum and Alternaria alternate
Is there any protocol?
Try this:
A novel 96-well gel-based assay for determining antifungal activity
against filamentous fungi by Anscha Mari Troskie, Nicolas Maré Vlok, Marina Rautenbach ⁎
Dear Mehdi Nikkhah
You can use broth dilution method according to M27A3 and M38A2 protocols.
For above mentioned methods, it is recommended to liophilize your essential oils and then use the powder for preparing accure concentrations of them.
See the attached file of a recent study on the antifungal activity of the latex of Euphorbia .
Thank you very much for your comments and pdf
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