The key step is cDNA synthesis. If you have total RNA then Qiagen has something miRScript RT II kit which converts all miRNA present in total RNA into miRNA-specific cDNA. That cDNA you can use with SYBr and amplify those cDNA with miRNA primer (of your interest). This kit is compatible with total RNA and SYBr. Otherwise, you will need to look at some other reagents and find compatibility with your stuff i.e your onetube PCR system should able to amplify miRNA. regular cDNA synthesis will work properly for miRNA detection.