Can I use sesame oil instead of corn oil to dilute JR-AB2-011 in DMSO for rats?

10 October 2022 0 8K Report

We are trying to dissolve a molecule soluble in DMSO to inject into the rats. Because we can use a maximum of 10% DMSO, the datasheet recommends the remaining to be 90 % corn oil. Due to the unavailability of corn oil in our lab, we are thinking of using sesame oil instead. What do you think, have you encountered any similar situation before?

Badges
Science topic

More Zainab Sabrie's questions See All

Is an encrypted dataset with Microsoft SEAL compatible with Cloudsim simulator??

Hello, I would like to do homomorphic encryption using Microsoft SEAL on my dataset and then use it as an input to the Cloudsim simulator to do clustering on. Is it possible and compatible with...

16 February 2021 3,915 1 View

What is the best Information Managment Software for higher education student ex Master student?

I need to build a tracking system for Post Graduate student. The aimed software helps admistative team to record deadlines, generate documents depending on pre input data in registeration phase...

26 December 2020 5,396 3 View

Ellagic acid dissolving?

Can anyone help me with dissolving ellagic acid? Its solubility is about 0.8 mg/ml, I try half this amount but it does not solve

23 December 2020 9,532 3 View

Can I see nanoparticles using optical microscope with stage micrometer?

I am working on Solid Lipid Nanoparticles I am doing MPhil research I am preparing a formulation My topic is Preparation AND Evaluation of Indomethacin Loaded Solid Lipid Nanoparticles for...

27 October 2020 9,496 4 View

Can you recommend a textbook for DSC technique?

Dear colleagues, can you please recommend me a good literature specific on the thermal analysis by DSC technique from the basic principles to advanced applications and interpretation of curves...

08 May 2020 2,988 10 View

Are children will be psychologicaly affected more than adults by the pandemic of Covid-19?

Fear and anxiety disorders are come in children and are directly affected affected by the environmental condition (parents reaction, peers, media).

05 April 2020 8,202 33 View

How to dilute an oil without changing its effect?

I want to have different concentrations of volatile oils, which of course do not mix with water. how it would be?

18 January 2020 6,502 4 View

How to mesh high pressure gradient regions?

I am simulating injector nozzel to see cavitations and want to validate Winklhofer results using Mohan's paper(attached in files). I simulated flow for simple geometry (attached in files) with...

15 January 2020 3,429 1 View

Please does anyone know how to add a tertiary Y-AXIS on a graph?

I am trying to plot keratinase activity, protein concentration and pH changes against incubation time in one graph but then does anyone know how to add three Y-AXIS in one graph? what I know of...

18 October 2019 7,876 3 View

What happens if negative SiV center in diamond is excited with a nonresonant laser light, say 514nm?

Exciting negative SiV center with 737 nm laser pulse, resonant with the D1 transition is generally done for measuring fluorescence. What if laser light of shorter wavelength is used , say 514 nm?

08 August 2019 7,314 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View