Can I use DNase I treated RNA for RNA seq?

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Samuel A. Logan

Yes, that should be fine. You'll need to quantify your RNA before sending it for RNAseq though. Run it through a BioAnalyser or something similar (nanodrop might be ok). The RNA needs to be a certain RIN (RNA Intregity Number). Illumina hi-seq, I think should be RIN of 7... Maybe different RIN for different methods... Illumina mi-seq, Bio Rad, Ion Torrent..

Good luck..

Muhammad Ehsaan

Thanks

Sara Mehrabi

Agree with Samuel.