Muhammad Ehsaan

3 Questions 5 Answers 0 Followers

Questions related from Muhammad Ehsaan

Lost of DNA/RNA concentration after two washes with 70% ethanol?

During nucleic acid extraction after precipitation with 100% ethanol, we wash the pellet with 70% ethanol to remove excess salts from the pellet. But during these washes, nucleic acid can also...

12 December 2019 5,354 7 View

How can I get rid of rRNA and tRNA from total extracted RNA?

I want to enrich mRNA and get rid of the rRNA and tRNA from the total extracted RNA from C. necator H16. any recommendations?

03 March 2019 2,916 1 View

Can I use DNase I treated RNA for RNA seq?

Hi, I have extracted total RNA and then treated with DNase I (NEB) to remove any DNA contamination and then heat inactivated. My questions are: 1. Can I use this RNA for RNA seq? OR 2. Do I need...

01 January 1970 7,310 3 View