Hello Ece Keskin,

Yes, you may use DLS instead of NTA, but it depends on your requirements.

DLS and NTA are similar techniques as they are based on the Brownian motion of and light scattering from the particle. But in practice though they are quite different techniques as DLS provides an intensity-based distribution and NTA produces a number-based distribution. So, you can use DLS to reveal the total sample composition, and NTA can be used to further resolve the peak of main interest in addition to providing the concentration result.

In other words, NTA will provide you with more data about the vesicles, while DLS will offer you additional information about a wider range of constituents present in the sample.

You may use DLS when the broadest size range needs to be covered, especially above 800nm and below 30nm, or when comparison of different batches is of interest. On the other hand, NTA may be considered when number-based high-resolution sizing is required, or when particle count or concentration is needed, or when accurate distribution shape or width is critical.

Therefore, based on your experimental requirement you can choose either DLS or NTA. NTA and DLS can be used as complementary methods as well.

Best.