Benzene extract of the plant does not dissolve in water.
https://www.researchgate.net/post/What_is_the_best_solvent_for_a_medicinal_plant_for_use_in_the_determination_of_LD50_if_extract_is_not_soluble_in_water2
various combinations are described
Yes you can
Yes it is feasible. The use of benzene raises my hair though
Yes, I think.
Thank you Professors.
you can use HPLC/CAD ,The solvent is methanol - water.
yes you can use tween 80
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