I am not familiar with the LAKH cell line, could you send the repository and cat #? Also, are you asking if you can plate 100,000 cells per well or 1 cell per well? The number of cells is based on size so without knowing more about the LAKH cell line, I cannot be absolutely sure. However, you cannot typically plate 100,000 cells per well at 96 well plates (usually only 1000-10,000), take a look at the manufacturers recommendation for max cell population per well. Also, typically you cannot accurately plate 1 cell per well as there is too much pipette deviation. The exception to this is single cell pipetting, common with embryos and large cells. But typically you cannot measure 1 cell with the MTT assay, so I assume you meant 100,000, in which case you probably need less cells.
To clarify, Daniel, Lakh is not a cell line. It is a measure (equivalent to 100,000) used only in the Indian sub-continent, as far as I am aware.
I agree with Daniel that you cannot perform assays with 100000 cells in 96 well plates. You could try titrating the number of cells from 1000 upwards and see where you get a good signal change between your negative and positive controls.
Dr. Janaka, thanks for clarifying. So lakh stands for 100,000 cells? Just curious.
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