Hi,
I'm working with a monster size protein and I try to resolve the endogenous version from a tagged version that usually blurres into one band when I develop my film.
One solution I found is to just run my 8% gel at 100-110V for 5-6h (minitank) but I was wondering if someone maybe has a migration calculator, for example maybe someone did it overnight at low voltage? But then, how low - 10V? 20V? 30V? Run it in an ice bath? Cold room?
Thanks!