I want to make a protein hydrolysate from Black Soldier Fly Larvae (BSFL) crude protein extract using the protease Bromelain. But, for reducing the cost in enzyme usage, can Bromelain be used for several times? How to reobtain the enzyme and for how many cycles? Is there any reference(s) about it? Thank you very much.
Steingrimur Stefansson
Felicia Tejo Hi, you could use bromelain as long as it is active. You can use the chromogenic synthetic substrate pGlu-Phe-Leu-p-nitroanilide to check its activity before and after use.
But bromelain is a thiol proteinase that has a free -SH group that can be oxidized by exposure to air.
In lieu of bromelain, you could use bovine chymotrypsin which has the same specificity as bromelain, is more stable and it is cheap.
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