Hey everyone

I am using the ddRADSeq method for my research question. I build my libraries (double indexed - Illumina index and inline barcode) and ran it on the NextSeq as test run. Now I want to load the library again to get more reads per sample.

The problem is that the bioinformatic analysis of my test run results in very less loci shared among just a few samples. So, I think my range of the size selection was to large. Can I run my libraries on a gel and repeat the size selection step? Does is cut the libraries when I use this method of size selection? Would you prefer a Pippin Prep?

Has anyone done that before with success?

I would be great if someone has an answer to that.

Thanks a lot and Happy Easter.

Julia