I cleaned my diatom samples using buffer 2%SDS in 0.1M EDTA. I suspend my diatom samples in 20 mL buffer inside 50 mL polypropylene centrifuge tube, heat at 95 degree for 10minutes in water bath and centrifuge at 2800 rpm for 10 minutes. These steps, i repeated 3 times. Final steps, wash at least 3 times. Cleaned samples in the polypropylene centrifuge tube, i suspend 10 mL 95% ethanol into the tube for storage prior to microscopy analysis.
My questions:
1. How long can I keep my cleaned diatom in ethanol at room temperature? Does the diatom dissolves?
2. Does the polypropylene tube give any side effects to my diatom samples?
3. Does the silica of my diatom dissolved in any of the cleaning steps i used?
Thank you.
Normally I keep it in a glass vial in the refrigerator it can take many years.
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