Can I find Echinococcus granulosus in blood of host when testing the blood serum or blood smear?

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What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

What are the new methods of staining??

what are the new methods of staining??

25 December 2020 4,030 3 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

I am working on COVID19 dataset??

I am working on COVID19 dataset, but, When I tried to convert the data.xlsx file to CSV file, I got an error. Also, i got an error when I upload the CSV file to WEKA. I tried to solve the problem...

05 May 2020 4,879 2 View

How can i define the USRP N210 on pc under windows 10?

How can i define the USRP N210 on pc under windows 10 and i used gnu radio 3.8.. i set the configuration and i make the ping and recived reply successfuly but i cannot define the device and an...

21 January 2020 1,442 2 View

What is the number of measurement required in Circulant and Toeplitz sensing matrix in general in term of N an M in compressive sensing?

what is the number of measurement M (the number of measurement vector that result from multiplication of sparse vector N with sensing matrix (NxM)) required when using Circulant and Toeplitz...

16 January 2020 9,666 2 View

Does Tobramycin have an amorphous structure?

I've been analyzing the crystallinity of Tobramycin by the XRD, it is supposed to have a crystalline structure, but when analyzed, it showed an amorphous structure

25 November 2019 5,145 1 View

I've been trying to test the antimicrobial activity of zinc oxide nanoparticles against P.aeruginosa. They exhibited no activity. Any one knows why?

the nanoparticles were purchased from sigma and they have a size range (35-50 nm). i tried to dissolve it in DMSO ( 5% of the nanoparticle solution) and i also tried to sonicate it for 30...

24 August 2019 9,678 10 View

Combustion Engineering second edition by Kenneth W. Ragland, Kenneth M. Bryden?

I looked for this book literally everywhere but I can't find it, dose anyone have a PDF copy of this book because I really need it...

01 April 2019 6,305 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View