Iam presently working on "cloning of protease enzyme from Geobacillus species". But to get protease sequences to amplify the gene from Geobacillus sp. this has been very problematic, because i could not get protease sequences from Geobacillus to design primers for PCR amplification. Most sequences I came across are from Bacillus species.How do I get enough sequences for degenerate primer design from Geobacillus or can I use sequences from Bacillus species for the primer design. My gene of interest is a thermostable protease from a thermophillic Geobacillus sp.My colleagues told me I have to get the sequences from Geobacillus that have been resolved and published and not from complete genome of the organisms. I have accessed the sequences from complete genome from NCBI. I want somebody to give a link. Thanks

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