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Questions related from Suleiman Allison
I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...
16 March 2019 9,490 3 View
I have just cloned and expressed a thermophilic alkaline serine protease in E.coli BL21(D3). The protease was expressed as a soluble protein (about 60%) with about 30kDa Mol. wt. (confirmed by...
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Iam presently working on "cloning of protease enzyme from Geobacillus species". But to get protease sequences to amplify the gene from Geobacillus sp. this has been very problematic, because i...
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I incubated my organism (thermophillic bacteria) on LB broth at 37 and 40 degrees centigrade for the purpose of extracting DNA from the organism but did not show any growth after 22hrs of...
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