Can i coupled the mannose with sepharose -6B resin

More Parthiban Ezhumalai's questions See All

In the Protein drug conjugation, How to calculate the Protein drug ratio by UV method?

I am performing the synthesis of a protein-drug conjugation. After the conjugation, how do I calculate the concentrations of the protein and drug using UV? Kindly provide examples of calculations.

17 August 2023 8,114 0 View

How to Select an Electrolyte for Supercapacitor?

When to use aqueous electrolyte and when to employ an acidic electrolyte for supercapacitor?

14 December 2022 8,830 3 View

Can anyone tell the protocol for the estimation of catalase using ammonium molybdate as indicator?

I need to estimate catalase using hydrogen peroxide, sodium potassium phosphate buffer.

08 March 2022 7,118 1 View

Can keeping whatever delivered, under the scorching heat helps to get rid of SARS-CoV-2?

In India we have tradition of keeping many groceries under the sun on terrace or exposed floor. Now due to covid situation, whatever delivered mainly and majorly being groceries,, we need to...

11 April 2020 6,619 3 View

How to safe guard oneself while travelling to poor air quality locations?

My city already has about 80 to 130 AQI and I might be shortly living in a worse city of AQI ranging from 100 to 180 AQI (Nanded, Raigarh). I have dust allergy. I could take mask, but apart from...

30 November 2019 639 10 View

Reg- Biochemical assay ?

We did biochemical assays like esterase and phosphatase (quantitatively) activity in the whole body homogenate of the larval extract. For that, first we have to estimate the protein concentration...

10 March 2019 9,453 2 View

Anna University Annex 1 journal suggestions?

Which current Anna university annexure 1 journal may i prefer for quick publication of my manuscript

30 November 2018 5,111 4 View

How to quantify Dibutyl Phosphate by HPLC?

I want to estimate the dibutyl phosphate in the synthetic saline waste water. I want to know the which column and HPLC parameters such as Mobile phase, Detector, flow rate, etc., to analyze the...

15 August 2018 6,860 2 View

please any one give valuable suggestion what are problems need to be address in content based classification and retrieval of image?

i am doing research in Content based image classification and retrieval using visual bag of feature. my doubt is how to do denoise the image in CBIR

03 June 2018 9,812 5 View

Now i am going to do antibacterial activity using crude plant extract so for that how do i make diffrent concentartion..

Now i am going to do antibacterial activity using crude plant extract so for that how do i make different concentration of 10,20,30ug/ml from stock 1mg/mL. but to my knowledge i am using following...

26 July 2017 259 4 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

Prevent sedimentation and maintain the suspension of powder particles in the resin ?

Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...

23 July 2024 1,672 0 View

Any relation between resin viscosity（mPa*s）and resin relative molecule weight？why the former is used in manufacture?

https://en.wikipedia.org/wiki/Viscosity

21 July 2024 3,089 4 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Recommendations for his-tag magnetic beads / resins?

Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...

16 July 2024 3,692 6 View

How to draw photon-magnon coupling color plot in origin?

Please help me in plotting the photon-magnon coupling plot as shown below

14 July 2024 1,632 1 View

What is the best way to get rid of unpleasant odour of acrylic emulsion beside redox post polymerization?

Even adding fragrance is not working

05 July 2024 741 2 View

How to solve the question of ansys fluent?

Regarding the system coupling issue where the update of the solution component fails, the system coupling update for fluid flow (Fluent) triggered an exception: "Update Solution Failed."

30 June 2024 7,894 1 View

Omar Gonzalez-Ortega

Yes you can, you could use epichlorohydrin

Steingrimur Stefansson

Hi Parthiban, you could also use D-Mannosamine available from Sigma and other companies.

It will couple to all amine reactive affinity resins.

Following Stefansson's comment you could oxidize the matrix with periodate to generate aldehyde groups that can be used to attach the mannosamine