Reg- Biochemical assay ?

We did biochemical assays like esterase and phosphatase (quantitatively) activity in the whole body homogenate of the larval extract. For that, first we have to estimate the protein concentration...

10 March 2019 9,348 2 View

please any one give valuable suggestion what are problems need to be address in content based classification and retrieval of image?

i am doing research in Content based image classification and retrieval using visual bag of feature. my doubt is how to do denoise the image in CBIR

03 June 2018 9,659 5 View

Now i am going to do antibacterial activity using crude plant extract so for that how do i make diffrent concentartion..

Now i am going to do antibacterial activity using crude plant extract so for that how do i make different concentration of 10,20,30ug/ml from stock 1mg/mL. but to my knowledge i am using following...

26 July 2017 165 4 View

How to remove excess organic ligands from QDs?

I am having problems in making completely dried QDs films. It always remains sticky. I think it is because of the excess organic ligands (Palmitic acid, TOP, Oleic acid). I purified the QDs by...

21 March 2017 1,787 4 View

How can I determine the right orientation of windings in a distributed winding stator?

I have a stator(PMSM) with distributed windings in it. I need to determine whether the windings are wound in the correct direction. Is there a method to do it? The stator has 144 slots with 2...

22 June 2014 8,492 2 View

What software can I use to draw unit cell structure?

I would like to draw unit cell structures such as wurtzite and cubic. Is there any free softwares to draw unit cells. Attached image is the model which I want to draw.

19 June 2014 9,677 37 View

Can anyone help with indexing a SAED ring pattern?

I have recorded the SAED pattern of my sample. It has a ring pattern and the scale bar is 5 1/nm. Please give me information about how to index SAED pattern using reciprocal scale bar.

02 February 2014 2,711 4 View

Dynamic Surface Electromyography (sEMG) - Processing techniques, analyzing methods and its important characteristics

I am trying to learn about the muscle characteristics of a particular neurodegenerative disease: 1) What kind of information (i.e muscle weakness, Spasticity, fatigue) should I look out for in...

14 January 2013 2,434 9 View

Is it possible to use EMG data, collected using Vicon motion capture system, in Matlab for signal processing?

I am working on Gait analysis. I need to do EMG signal processing and it looks like Matlab is a good option for that. But I have collected data through Vicon motion capture systems and is it...

08 November 2012 4,937 12 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View